IRON Laboratories     PJLA

ISO/IEC 17025:2017 Accredited

Marihuana Potency Analysis by
High Performance Liquid Chromatography

Testing Accreditation #: 77802 Test Certificate #: 127288-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: CBN
Type: Tincture
Method: FE04U HPLC-UV

Test Conditions

Prepsheet ID#: MIP201022
Scale: XS205-MI2
Temp: 20.8 °C
Baro Pressure: 985.6 hPa
Analyst: KEB
Technician: ANJ

Sample ID#: 127288

Harvest/Process Date: 10/23/2020
Serving Size (g): 9.2
Date Received: 10/23/2020
Test Date: 10/22/2020
Valid Through: 10/23/2021
Report Issued: 10/23/2020

Sample #127288
Sample #127288 Sample #127288
Test
Compounds
THC THCA CBD CBDA CBN CBG* CBC* THCV* CBDV* Total
Cannabinoids*
Total THC Total CBD Calc Max Total
Cannabinoids*
Amount (%) 0.3 N/D 7.0 0.1 7.0 0.2 0.3 0.1 0.1 14.9 0.3 7.0 7.3
Amount (mg/g) 2.7 N/D 69.7 0.5 69.8 1.6 3.1 0.7 1.2 149.3 2.7 70.1 149.3
Amount per Serving (mg) 24.8 N\D 641.2 4.6 642.2 14.7 28.5 6.4 11.0 1,373.6 Serving Size~ (g): 9.2
LOQ (mg/g) 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08   %Decarb. THC CBD
±%RPD 0.13 0.07 0.81 0.77 1.35 1.49 0.51 0.82 0.85 100% 99

Serving size = 10 mL = 9.2 grams (declared).

LOQ = Limit of Quantitation; %RPD = Relative Percent Deviation; %RSD = Relative Standard Deviation; N/D = Not Detected

*Designates values that are not currently included in the accredited scope of Iron Laboratories.

*** Designates tests that use the method FE-45. FE-45 is performed using AOAC 966.02 and 32.004-32.009. FE-45 has relative expanded (k=2) uncertainties of 1.098% for moisture, 1.754% for water activity for unprocessed plant materials, and 13.102% for water activity for infused products. Vitamin E acetate analysis has a relative expanded (k=2) uncertainty of 18.614%.

Total THC and CBD is the calculated sum of THC or CBD and the amount of THC or CBD derived from THCA or CBDA, respectively. These values are calculated by applying a molar correction factor of 0.877 to the THCA or the CBDA value. Calc Max Total Cannabinoids is the sum of Total THC, Total CBD, CBN, CBG, CBC, THCV, and CBDV.

%Decarb. THC and CBD refer to the percentage of THC or CBD relative to THCA or CBDA, respectively.

This sample has not undergone random sampling and has not been tested for compliant state, batch representative testing. These results should therefore be used for research and development or quality control purposes only. Results apply to the sample as received.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.

KEB - LM Iron laboratories KEB - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2017 Testing Laboratory laboratory accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

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IRON Laboratories     PJLA

ISO/IEC 17025:2017 Accredited

QC Report

Testing Accreditation #: 77802 Test Certificate #: 127288-001
Client Name, Sample Details

Holistic Healing Tim/RoseAnn
Troy, MI 48083
Sample: CBN
Type: Tincture
Method: FE04U HPLC-UV

Test Conditions

Prepsheet ID#: MIP201022
Scale: XS205-MI2
Temp: 20.8 °C
Baro Pressure: 985.6 hPa
Analyst: KEB
Technician: ANJ

Sample ID#: 127288

Harvest/Process Date: 10/23/2020
Serving Size (g): 9.2
Date Received: 10/23/2020
Test Date: 10/22/2020
Valid Through: 10/23/2021
Report Issued: 10/23/2020

Sample #127288
Target Compound Name Method Blank (μg/g) LCS Spike (μg/g) LCS Amount (μg/g) Percent Recovery (%) LCS LCS Duplicate Amount (μg/g) Percent Recovery (%) LCSD Relative Percent Difference (%) QC Flag
Cannabidivarin (CBDV) 0 5.441652173 5.79332254 106.46 5.744022459 105.56 0.85
Cannabidiolic Acid (CBDA) 0 4.699453608 5.033918625 107.12 4.995307418 106.30 0.77
Cannabigerol (CBG) 0 5.380770706 5.769293997 107.22 5.683855336 105.63 1.49
Cannabidiol (CBD) 0 5.406193141 5.691117412 105.27 5.645386672 104.42 0.81
Δ9-Tetrahydrocannabivarin (THCV) 0 5.465090335 5.871802936 107.44 5.823973669 106.57 0.82
Cannabinol (CBN) 0 5.333650949 5.745407635 107.72 5.668109443 106.27 1.35
Δ9-Tetrahydrocannabinol (THC) 0 5.3669249 5.823955077 108.52 5.816445193 108.38 0.13
Cannabichromene (CBC) 0 5.320539056 5.734030357 107.77 5.704932365 107.22 0.51
Tetrahydrocannabinolic acid (THCA) 0 4.831134766 5.137559229 106.34 5.13394981 106.27 0.07

N.D. = Not Detected

LR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) Low recovery should be scrutinized for possible fail as it could indicate more compound present than is detected.

I = indicates that an amount of an interfering compound greater than the methods limit of detection was detected in the method blank sample. May indicate contamination of analytical system or consumables.

Q = indicates that the relative percent diference of two identicly prepared Matrix Spike samples for a target analyte was greater than 20%

HR = indicates compound recovery of matrix spike was outside the methods acceptable limits. (70-130%) high recoveries should be scrutinized for passing as more compound may be detected than is actually present in the sample.

This sample has not undergone random sampling and has not been tested for compliant state, batch representative testing. These results should therefore be used for research and development or quality control purposes only. Results apply to the sample as received.

This certificate shall not be reproduced except in full, without written approval of Iron Laboratories, LLC.

KEB - LM Iron laboratories KEB - LM

Iron Laboratories, LLC is an ISO/IEC 17025:2017 Testing Laboratory laboratory accredited by (PJLA) Perry Johnson Laboratory Accreditation, Certificate No. 77802

Tested by Iron Laboratories Michigan, 1825 E. West Maple Walled Lake, MI 48390

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